Figure 6
From: CRISPR/Cas9-mediated gene knockout in the mouse brain using in utero electroporation
The effects of pX330-Satb2-2129 on axonal projection patterns of layer 2/3 neurons.
Layer 2/3 neurons were co-transfected with pCAG-EGFP and pX330-Satb2 (2.5 mg/ml) using in utero electroporation at E15.5. Coronal sections were prepared at P4 and stained with anti-EGFP antibody (green) and Hoechst 33342 (blue). (a) Upper panels are low magnification images of coronal sections. Areas indicated by an arrow and an arrowhead in the upper panels are enlarged and shown in the lower panels. Note that EGFP-positive axons extended to the contralateral cortex in control animals (arrows), whereas they were markedly decreased by pX330-Satb2-2129 (arrowheads). Scale bars = 1 mm (upper) and 500 μm (lower). (b) Upper panels are low magnification images of coronal sections located more posterior to the sections in (a). Areas indicated by an arrow and an arrowhead in the upper panels are enlarged and shown in the lower panels. Note that no EGFP-positive axons were found in the internal capsule in control animals (arrows), whereas EGFP-positive axons were found in the internal capsule of animals transfected with pX330-Satb2-2129 (arrowheads). Scale bars = 1 mm (upper) and 500 μm (lower).
