Table 1 Mg2+ and Ca2+ doses in 10 mM HEPES buffer a .

From: Calcium and Magnesium Ions Are Membrane-Active against Stationary-Phase Staphylococcus aureus with High Specificity

M2+ (mM)

HEPES (mM)

NaCl (mM)

Sucrose (mM)

0

10

170

0

5

10

155

0

10

10

140

15

20

10

110

45

40

10

50

105

  1. aHEPES, NaCl, and sucrose were supplemented to help keep the pH, final ionic strength and final osmolarity constant at 7.4, 170 mM, and 325–340 RT (where R is the gas constant and T is ambient temperature, and ideal solutions are assumed), respectively. Same buffers were used for all experiments through this work unless specified otherwise.