Figure 1
From: Atlas Toolkit: Fast registration of 3D morphological datasets in the absence of landmarks
Group-wise 3D object registration using Atlas Toolkit.
(a) Group-wise registration of multiple 3D objects is achieved by the sequential and iterative registration of orthogonal 2D projections. (b) For each orthogonal plane, the 2D projections are registered group-wise by determining the mean transformation coefficients resulting from multiple pair-wise registrations. (c) 3D reconstruction of three superimposed Optic Vesicle objects (red, green and blue, respectively) segmented from stage HH12 chick embryos before group-wise 3D registration. (d) Intersection of the three Optic Vesicle objects shown in (c) before group-wise 3D registration. (e) Intersection of the three Optic Vesicle objects shown in (c) after group-wise 3D registration using Atlas Toolkit. (f) Graph quantifying the convergence of 7 simulated landmarks in three manually deformed objects, expressed as mean pair-wise distance in microns (μm), with increasing iteration numbers. (g) 3D reconstruction showing the initial distribution of 7 simulated landmarks in the three manually deformed objects (red, green, blue). (h) 3D reconstruction showing the convergence of landmarks in (g) following registration using Atlas Toolkit. (i) Relationship between mean volumetric overlap and increasing iteration number, for three or 18 real Optic Vesicle objects. (j) Relationship between mean volumetric overlap and increasing numbers of real Optic Vesicle objects, for one or six iterations.
