Figure 3
From: Atlas Toolkit: Fast registration of 3D morphological datasets in the absence of landmarks
Hierarchical clustering of ‘Optic Vesicle-space’ reveals multiple domains of divergent combinatorial cell signalling and predicts spatially distributed Gene Regulatory Network states.
(a) Dendrogram showing the hierarchical relationship of six Optic Vesicle zones with divergent signalling profiles. (b) Heat map showing the mean nuclear signal levels (normalised to background) for each of the identified clusters/zones. (c) Heat map showing mean nuclear Pax6 level (normalised to background) for each cluster/zone. (d–i) 3D reconstruction of each of the six zones shown within the consensus Optic Vesicle morphology. (d) Zone 1, apical/medial view. (e) Zone 2, basal/dorsal-lateral view. (f) Zone 3, apical/medial view. (g) Zone 4, basal/dorsal-lateral view. (h) Zone 5, apical/medial view. (i) Zone 6, basal/dorsal-lateral view. (j–l) Three different views of the wholly reconstructed consensus Optic Vesicle morphology, colour-coded to display the six zones in (d–i). (j) Basal/dorsal-lateral view. (k) Apical/medial view. (l) Basal/caudal-lateral view.
