Table 1 Validation of ISH results using PCR based methods.

From: Evidence of disrupted high-risk human papillomavirus DNA in morphologically normal cervices of older women

 

Luminex Positive N = 27

Luminex Negative N = 30

Chi Square (pearsons)

p Value

relative risk

95% Confidence Interval

Ventana Positive

8

0

10.34

0.0013

  

Ventana Negative

19

30

    
 

Q-PCR Positive N = 24

Q-PCR Negative N = 24

    

Ventana Positive

18

5

14.11

<0.0001

3.3

1.6–6.7

Ventana Negative

6

19

    
  1. For the Luminex assay, 76 samples were tested for HR-HPV types and 57 provided a usable result which could be compared to Ventana ISH results. When we compared the overlap between Ventana positive and Luminex positive samples we found 8/27 cases were HPV positive using both methods. When we compared the overlap between Ventana negative and Luminex negative samples we found 30/30 cases were HPV negative using both methods. When we compared the overlap between Ventana negative and Luminex positive samples we found 19/27 were negative using Ventana but positive using Luminex. When we compared the overlap between Ventana positive and Luminex negative samples we found 0/30 were positive using Ventana but negative using Luminex. Using a chi square test (pearsons) we found Ventana ISH positive samples were significantly more likely to test positive for HPV using Luminex (p = 0.0013). For the nested E6 Q-PCR assay, 55 samples were tested for HPV 16 and 48 provided a usable result which could be compared to Ventana ISH results. When we compared the overlap between Ventana positive and Q-PCR positive samples we found 18/24 cases were HPV positive using both methods. When we compared the overlap between Ventana negative and Q-PCR negative samples we found 19/24 cases were HPV negative using both methods. When we compared the overlap between Ventana positive and Q-PCR negative samples we found 5/24 were positive using Ventana but negative using Q-PCR. When we compared the overlap between Ventana negative and Q-PCR positive samples we found 6/27 were negative using Ventana but positive using Q-PCR. Using a chi square test (pearsons) we found Ventana ISH positive samples were also significantly more likely to test positive for HPV using Q-PCR (p = < 0.0001).
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