Figure 5: Circular dichroism spectroscopy of domain AISO.
From: Ribosomal small subunit domains radiate from a central core
(a) Mg2+ titration of domain AISO rRNA (solid black), the C18A mutant of domain AISO (dashed blue), and domain AISO rRNA with helix 28 excised (dotted red). Mg2+ concentration is plotted versus the intensity of the diagnostic CD peak (265 nm). (b) CD spectra of the same series of RNAs in the presence of 1.0 mM Mg2+. The outbox shows a close-up of the 265 nm peak. Initial rRNA samples were depleted in Mg2+ ions.
