Figure 1: Plin2 mRNA but not protein levels are increased during adipocyte differentiation of MEFs and SVCs.

(A) MEFs were subjected to adipocyte differentiation as described in the Materials and Methods and the cells were harvested every 2 days. Quantitative RT-PCR was performed and each mRNA level was determined and normalized by 18s rRNA levels. These data represent the means ± S.D. The assay was performed in triplicate. (B) Differentiated MEFs were harvested every 2 days and each protein level was determined using Western blot analysis. (C) After 7 days of differentiation, MEFs were treated with 10 μM MG-132, 50 μM ALLN, 10 μM Lactacystin, or 10 μM E-64d for 12 h. Plin2 and actin proteins from whole cell extracts were detected using Western blot analysis. (D) SVCs were subjected to adipocyte differentiation as described in the Materials and Methods and cells were harvested every 2 days. Quantitative RT-PCR was performed and each mRNA level was determined and normalized by 18s rRNA levels. These data represent the means ± S.D. The assay was performed in triplicate. (E) Differentiated SVCs were harvested every 2 days and each protein level was determined using Western blot analysis. (F) After 7 days of differentiation, SVCs were treated with 10 μM MG-132 for 12 h. Plin2 and actin proteins from whole cell extracts were detected using Western blot analysis.