Figure 3: Modulation of phagocytosis following activation of TLR 4 and TLR 2 in morphine treated primary macrophages is naltrexone reversible. | Scientific Reports

Figure 3: Modulation of phagocytosis following activation of TLR 4 and TLR 2 in morphine treated primary macrophages is naltrexone reversible.

From: Differential effects of gram-positive and gram-negative bacterial products on morphine induced inhibition of phagocytosis

Figure 3

Fluorometric quantification of phagocytosis by J774 macrophages. Naltrexone (10 μM) was added 2 hr prior to overnight morphine (1 μM) treatment of primary macrophages from WT mice. (A) LTA (10 μg/ml) and (B) LPS (50 ng/ml) were added 4hr before addition of OPDex beads. Phagocytosis was allowed for 60 min and cells were analyzed via fluorometric analysis and expressed as % control (Phagocytic index = FITC (RFU)/DAPI(RFU)). Data quantified as % from vehicle control, mean ± standard error. Significance was determined using student’s t-test (***p < 0.0001, **p< 0.001, *p < 0.05).

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