Figure 5: Membrane targeted miniSOG-mediated cell killing is cell autonomous.
From: Highly efficient optogenetic cell ablation in C. elegans using membrane-targeted miniSOG
(a,b) GABAergic motor neurons (Punc-25-GFP) are intact while cholinergic motor neurons (Pacr-2-mCherry) degenerate 4 h after blue light illumination in Punc-17β-PH-miniSOG transgenic animals. L4 stage (a) and adult (b) animals were analyzed. White arrows indicate degenerated cholinergic neuron fragments. Yellow arrow indicates mCherry debris in the coelomocytes. Scale, 10 μm. (c) Muscle cells (marked by Pmyo-3-GFP(ccIs4251)) are intact while cholinergic motor neurons (Pacr-2-mCherry) degenerate 4 h after blue light illumination in Punc-17β-PH-miniSOG transgenic animals. Adult animals were analyzed. White arrows indicate cholinergic neuron fragments. Scale, 10 μm.
