Figure 6: The Q103L variant miniSOG enhances cell ablation efficacy.
From: Highly efficient optogenetic cell ablation in C. elegans using membrane-targeted miniSOG
(a) Quantitation of paralysis in animals expressing epidermal PH-miniSOG and PH-miniSOG (Q103L), immediately after blue light illumination (2 Hz) for the indicated times. (b) Onset of paralysis in animals expressing epidermal PH-miniSOG or PH-miniSOG (Q103L), after 1 min 2 Hz blue light illumination. (c) Quantitation of paralyzed PH-miniSOG and PH-miniSOG(Q103L) animals immediately after continuous blue light illumination, for the indicated times. (d) Onset of paralysis in animals expressing epidermal PH-miniSOG and PH-miniSOG (Q103L) animals after 20 sec continuous blue light illumination. (e) Green fluorescence images of Pcol-19-PH-miniSOG(WT) and Pcol-19-PH-miniSOG(Q103L) transgenic animals, identical exposures. Scale, 250 μm. (f) Quantitation of green fluorescence intensity in panel e. ***P < 0.001, t-test. Numbers are the animals that were analyzed. (g) Quantitation of Unc animals immediately after 2 Hz blue light illumination, for the indicated times. PH-miniSOG and PH-miniSOG(Q103L) were expressed under the control of unc-17β promoter. (h) Quantitation of Unc animals after 0.5 and 1 min continuous blue light illumination.
