Figure 4 | Scientific Reports

Figure 4

From: Biochemical characterization of extra- and intracellular endoxylanse from thermophilic bacterium Caldicellulosiruptor kronotskyensis

Figure 4

Hydrolytic patterns of Xyn10A-WT, Xyn10A-TM1, Xyn10A-TM2 and Xyn10B.

(A) TLC analysis of the hydrolytic products of BWX, OSX and XOS. (B) HPLC analysis of the released xylose from BWX, OSX and XOS. (C) Specific activity of Xyn10A-WT, Xyn10A-TM1, Xyn10A-TM2 and Xyn10B with BWX and XOS at 55 °C, pH 7.0. Each of purified Xyn10A-WT, Xyn10A-TM1, Xyn10A-TM2 and Xyn10B was incubated with BWX, OSX and XOS at 55 °C for 16 h in 200 μl Tris buffer (pH 7.0, 50 mM Tris-HCl, 150 mM NaCl), respectively. The substrate XOS is consisted of xylobiose, xylotriose, xylotetraose, xylopentaose and other xylo-oligosaccharides. X1, xylose; X2, xylobiose; X3, xylotriose; X4, xylotetraose.

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