Figure 2

BIRC3 is up-regulated in therapy resistant GBMs.

(a) BIRC3 gene is expressed at higher level in recurrent GBMs than in newly diagnosed GBMs. GBM samples were collected from newly diagnosed and recurrent GBM patients. Total RNA was extracted and cDNA was synthesized. BIRC3 gene expression was measured by RT-PCR analysis. (n = 7–8, p < 0.05). (b) TMZ drug resistant U87 cells were developed with gradual increase in TMZ concentration. Cells were harvested and total RNA was extracted and cDNA was synthesized. BIRC3 gene expression was measured by RT-PCR between WT-U87 and 50 μM TMZ resistant U87. (n = 3 independent experiments, p < 0.0001). (c) U87 cells were irradiated with increasing doses of X-ray. Cells were harvested and total RNA was extracted and cDNA was synthesized. BIRC3 gene expression was measured 24 hours later by RT-PCR. (n = 3 independent experiments, p < 0.05). (d) BIRC3 protein expression was increased upon TMZ treatment or X-ray irradiation in U87 cells. X-ray irradiated cells were harvested 24 hours after irradiation and Western blot analysis was performed using BIRC3 and actin antibodies. Data are representative of three independent experiments. p < 0.05.