Figure 3: SOCS3 mediates GW4064 effects on leptin signalling molecules in MCF-7 breast cancer cells. | Scientific Reports

Figure 3: SOCS3 mediates GW4064 effects on leptin signalling molecules in MCF-7 breast cancer cells.

From: Activated FXR Inhibits Leptin Signaling and Counteracts Tumor-promoting Activities of Cancer-Associated Fibroblasts in Breast Malignancy

Figure 3

(a) Total RNA was extracted from MCF-7 cells treated with GW4064 as indicated, reverse transcribed and cDNAs were subjected to PCR using primers specific for SOCS3 or 36B4 (internal control). NC: negative control. Immunoblotting analysis for SOCS3 expression in total protein extracts from MCF-7 cells treated with GW4064 as indicated (b) or from MCF-7 cells transiently transfected with either empty vector (e.v.) or FXR dominant negative plasmid (FXR-DN) and treated with GW4064 (c) or from MCF-7 cells transiently transfected with either a control siRNA (C siRNA) or a siRNA targeted for the human SOCS3 RNA sequence (SOCS3 siRNA) (d). (e) Levels of phosphorylated (p) MAPK and Survivin were evaluated in protein extracts from MCF-7 cells transfected and treated as indicated by immunoblot analysis. β-Actin was used a loading control. The histograms represent the mean ± SD of three separate experiments in which band intensities were evaluated in terms of optical density arbitrary units (OD) and expressed as percentage of vehicle-treated samples which were assumed to be 100%. n.s. = non significant; *p < 0.05.

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