Figure 4: GW4064 effects on CAF conditioned media-induced growth and motility in breast cancer cells. | Scientific Reports

Figure 4: GW4064 effects on CAF conditioned media-induced growth and motility in breast cancer cells.

From: Activated FXR Inhibits Leptin Signaling and Counteracts Tumor-promoting Activities of Cancer-Associated Fibroblasts in Breast Malignancy

Figure 4

(a) Soft agar growth assays in MCF-7 and MDA-MB 231 cells treated with CAF-derived conditioned media (CAF-CM) in the presence or absence of GW4064. (b) Wound-healing assays in cells treated as indicated. Fields were photographed immediately after wounding (inset, time 0) and 24 or 12 hours later for MCF-7 and MDA-MB-231 cells, respectively. Upper panel, representative images from each condition are shown. Lower panel, the histograms represent the relative percentage of wound closure calculated by image analysis using Scion Image software. (c) Matrigel invasion assays in cells treated as indicated. The migrated cells were DAPI-stained, counted and images were captured at 10X magnification. Typical well for each condition is shown. The data represent the mean ± SD of three independent experiments, each performed in triplicate. *p < 0.05.

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