Figure 5: Impact of GW4064 treatment on tumor growth of MCF-7 and MCF-7/CAF xenografts. | Scientific Reports

Figure 5: Impact of GW4064 treatment on tumor growth of MCF-7 and MCF-7/CAF xenografts.

From: Activated FXR Inhibits Leptin Signaling and Counteracts Tumor-promoting Activities of Cancer-Associated Fibroblasts in Breast Malignancy

Figure 5

(a) MCF-7 cells were injected alone or coinjected with CAFs (MCF-7/CAFs) subcutaneously into nude mice (5 mice/each group). GW4064 treatment (30 mg/kg/day) was started when tumor size reaches to 100 mm3 (day 0, MCF-7/CAFs group; day 12, MCF-7 group) and delivered daily to the animals by i.p. injection. Relative tumor volume (RTV) was calculated from the following formula: RTV = (Vx/V1), where Vx is the tumor volume on day X and V1 is the tumor volume at initiation of the treatment for each group. Y axis: the mean and ± SD of the RTV. *p < 0.05, GW4064-treated versus vehicle-treated animals. (b) Representative Hematoxylin and Eosin (H&E) and (c) Azan trichrome stained histologic images of MCF-7/CAF xenograft tumors (20X). (d) Human αSMA (left panel) and Cytokeratin 18 (right panel) immunohistochemical staining of MCF-7/CAF xenograft tumor sections. Scale bars = 25 μm.

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