Figure 5: Effects of RIPK1, RIPK3 and MLKL on foam cell necroptosis induced by serum starvation. | Scientific Reports

Figure 5: Effects of RIPK1, RIPK3 and MLKL on foam cell necroptosis induced by serum starvation.

From: 5-Aminolevulinic Acid-Mediated Sonodynamic Therapy Inhibits RIPK1/RIPK3-Dependent Necroptosis in THP-1-Derived Foam Cells

Figure 5

(a,e) and (i) THP-1-derived foam cells were transfected with siRNA targeting MLKL, RIPK1 and RIPK3, as indicated. The foam cell death rate was measured using an LDH assay, separately. (n = 6; *P < 0.05 and ***P < 0.001 compared to the siRNA-negative group). (b,f) and (j) MLKL phosphorylation and oligomerization was detected using an MLKL phosphospecific antibody. GAPDH was used as a loading control (bottom panel). (c,g) and (k) The lysates from cells transfected with or without siRNA were immunoprecipitated with an antibody against RIPK1, followed by western blotting analysis with an antibody against RIPK3. The input whole-cell lysates were probed with RIPK1 and RIPK3 antibodies. (d,h) and (l) Confocal microscopy images showing the distribution and colocalisation of RIPK1 and RIPK3 in serum-starved foam cells transfected or not with siRNA (scale bars = 8 μm). The data represent means ± S.E.M.

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