Figure 8: The effects of ZDEVD on caspase-8 activation and the anti-necroptotic effect of ALA-SDT in foam cells. | Scientific Reports

Figure 8: The effects of ZDEVD on caspase-8 activation and the anti-necroptotic effect of ALA-SDT in foam cells.

From: 5-Aminolevulinic Acid-Mediated Sonodynamic Therapy Inhibits RIPK1/RIPK3-Dependent Necroptosis in THP-1-Derived Foam Cells

Figure 8

(a) The changes of protein level of cleaved caspase-3/8 in foam cells at the different time point after ALA-SDT. (b) The dynamics of activated caspase-3/8 activity after ALA-SDT. (n = 4; *P < 0.05 and ***P < 0.001 compared to the control group). (c) Pretreatment with a caspase-3-specific inhibitor, z-DEVD-fmk (ZDEVD, 20 μM) inhibits the increased protein levels of cleaved caspase-8 in ALA-SDT treated foam cells. (d) ZDEVD blocks the increased activity of caspase-8 in ALA-SDT treated foam cells. (n = 5; ***P < 0.001 compared to the control group, ###P < 0.001 compared to the SDT group). (e) ZDEVD reverses the anti-cell death effect of ALA-SDT, analyzed with flow cytometry (left panel). The right panel shows the quantification data of flow cytometry analysis. (n = 3; *P < 0.05 and **P < 0.01 compared to the control group, #P < 0.05 compared to the SDT group). (f) ZDEVD stabilizes the RIPK1-RIPK3 complex in ALA-SDT treated foam cells. (g) ZDEVD blocks the decreased pMLKL oligomerization levels in ALA-SDT treated foam cells. (h) A proposed model of the ALA-SDT induced anti-necroptotic effect. The data represent means ± S.E.M.

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