Figure 6
Sensitivity of TRPV1 to pH 6 is altered in the T406D mutant.
pH 6, 0.3 μM and 3.3 μM capsaicin-induced currents of TRPV1WT and TRPV1T406 mutants in the absence of extracellular Ca2+. Application of Ringer’s solution at pH 6 or 0.3 μM capsaicin induces currents in TRPV1WT (a) and TRPV1T406A (b) receptor variants. TRPV1T406D expressing cells respond to pH 6 or 0.3 μM capsaicin only after priming the cells with 3.3 μM capsaicin (c). Ratio between first and second response indicates sensitization (<1) or desensitization (>1) during 3.3 μM capsaicin priming (d). Analysis of n = 10–15 independent measurements shows that TRPV1T406D is sensitized by priming with 3.3 μM capsaicin (unpaired WR-test, p < 0.05).
