Figure 1: Graphical summary of the three nuclear phylogenomic datasets used to infer relationships in the Rhizoplaca melanophthalma species group.

The relative sizes of the nuclear datasets are shown in comparison to the estimated size of the complete R. melanophthalma reference genome (specimen ‘mela_REF’) and the reference assembly. The ‘RealPhy’ dataset was constructed by mapping reads from all sampled specimens to the R. melanophthalma reference assembly using RealPhy v1.124. The ‘CEGMA’ matrix included 430 core eukaryotic genes and associated introns, each with an average length of ca. 5500 base pairs/CEG. The ‘100, 1 Kb loci’ dataset was comprised of 100, 1 kilobase loci selected from each of the 100 largest contigs in the R. melanophthalma reference genome assembly.