Figure 6: PANDAR controls p16INK4A expression through regulating the recruitment of Bmi1 to its promoter. | Scientific Reports

Figure 6: PANDAR controls p16INK4A expression through regulating the recruitment of Bmi1 to its promoter.

From: LncRNA PANDAR regulates the G1/S transition of breast cancer cells by suppressing p16INK4A expression

Figure 6: PANDAR controls p16INK4A expression through regulating the recruitment of Bmi1 to its promoter.

(a) MCF-7 cells were lysed and the clarified lyses were subjected to RIP with indicated antibodies. The enrichment of indicated lncRNAs was evaluated with qRT-PCR. (b) MCF-7 cells stably silencing PANDAR were transfected with p16INK4A promoter (p16 promoter-wt) or its mutant deleting Bmi1 response element (p16 promoter-mut) for 24 h and the luciferase activity were evaluated. (c) MCF-7 cells were transiently transfected with indicated siRNAs for 48 h. Endogenous ChIP was performed with BMI1 or isotopic IgG and the enrichment of bound p16INK4A pro moter was evaluated with qRT-PCR. The results are expressed as the mean ± SD; n = 3, **p < 0.01, *p < 0.05.

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