Figure 4: Real-time qPCR to measure procyclin expression levels.
From: A new approach to chemotherapy: drug-induced differentiation kills African trypanosomes
GUSone bloodstream forms were exposed for 24 hours to the 5 DIP compounds DIP-01, DIP-02, DIP-03, DIP-07 and DIP-19 and to CA at 37 °C. Procyclin mRNA levels were determined by qPCR. Among the standard trypanocidal drugs only melarsoprol induced expression of procyclin mRNA above background level. Error bars show standard deviations for technical triplicates.
