Figure 5: Overview of the screening procedure to identify compounds triggering differentiation.
From: A new approach to chemotherapy: drug-induced differentiation kills African trypanosomes
7,495 compounds were screened at two concentrations in the GUS assay. 243 compounds induced GUS with a S/B >2-fold, of which 212 available compounds were subjected to dose-response analyses in the GUS and viability assays. 30 compounds induced GUS activity S/B >3-fold of which 28 compounds were available and highly pure. Five compounds were further analysed by flow cytometry (loss of VSG) and real-time quantitative PCR (induction of procyclin mRNA).
