Table 1 Mutant clone ratios detected by ddPCR.

From: Ultra–sensitive droplet digital PCR for detecting a low–prevalence somatic GNAQ mutation in Sturge–Weber syndrome

Mut clone ratios

Mut copy number *

Mut drop number

Wt drop number

ACD

FA (%)

FA range (%)

10%

144

101

707

16444

12.3

14.5–10.0

10%

166

114

754

16252

12.9

15.1–10.7

10%

150

103

1046

16196

8.7

10.3–7.1

5%

74

50

771

15951

6

7.6–4.3

5%

74

49

714

15547

6.3

8.0–4.6

5%

66

48

1050

16941

4.2

5.4–3.1

1%

14.4

9

681

14684

1.3

2.1–0.4

1%

10.2

7

729

16037

0.9

1.6–0.2

1%

16

11

1182

16282

0.9

1.4–0.4

0.50%

8.6

5

576

13723

0.8

1.6–0.1

0.50%

4.6

3

552

15250

0.5

1.2–0

0.50%

8.8

6

1031

16030

0.56

1.02–0.1

0.25%

5

3

679

14038

0.43

0.95–0

0.25%

3.6

2

631

12837

0.31

0.78–0

0.25%

4.2

3

1104

16872

0.26

0.58–0

0.10%

0

0

772

16230

0

0–0

0.10%

1.4

1

788

16400

0.12

0.42–0

0.10%

3.4

2

975

13931

0.2

0.50–0

  1. *: Calculated copy number by Quantasoft, Mut: mutant, Wt: wild–type, drop: droplet, ACD: accepted droplets, FA: fractional abundance, PFA: Poisson fractional abundance.