Table 3 Sequences of primers and probes.

From: Ultra–sensitive droplet digital PCR for detecting a low–prevalence somatic GNAQ mutation in Sturge–Weber syndrome

Primer information

Primer and probe sequences

PCR primers amplifying a 178-bp fragment containing a GNAQ mutation which was cloned into TA vector.

5′-ATTGTGTCTTCCCTCC−3′ (forward)

5′-GGTTTCATGGACTCAG−3′ (reverse)

ddPCR primers amplfying a 114-bp fragment containing a GNAQ mutation

5′-CCTGCCTACGCAACAAGAT−3′ (forward)

5′-AGGTTTCATGGACTCAGTTACTAC-3′ (reverse)

LNA probes

5′(HEX)−TGGGGAC + T + C + GAAC-3′(IABkFQ) (wild−type)

5′(HEX)−TGGGG + AC + T + T + GAAC-3′(IABkFQ) (mutant)

PNA probe (wild type)

5′-GGGACTCGAACTCTA-3′

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