Figure 4 | Scientific Reports

Figure 4

From: RETRACTED ARTICLE: Overexpression of miR-499-5p inhibits non-small cell lung cancer proliferation and metastasis by targeting VAV3

Figure 4

miR-499-5p directly targets VAV3.

(A) miR-499-5p target site resides at 383–389 of the VAV3 3′-UTR are conserved in different species. (B) VAV3 expression was negatively regulated by miR-499-5p at mRNA level. (C) Western blot analysis also showed miR-499-5p negatively regulated VAV3 expression. (D) Luciferase reporter assay for direct targeting of the 3′-UTR of VAV3 by miR-499-5p. Luciferase reporters with wild-type or mutant miR-499-5p target sequences of VAV3 were transfected into vector control or miR-499-5p stably infected A549 cells and luciferase activity was measured according to the manufacturer’s protocol. (E) Inverse correlation of miR-499-5p and VAV3-protein was observed in NSCLC samples and mRNA expression of VAV3 was high or low in low- or high-miR-499-5p human NSCLC samples. (F) Overall survival of NSCLC patients with low and high VAV3 expression. Error bars represent the mean ± s.d. of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001.

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