Figure 3

(a): Schematic of Brugia malayi (Bm) SCID mouse PD model with oral dosing regimens (MK: mg/Kg). (b): Effect on Wolbachia depletion following indicated human bioequivalent dosing of doxycycline (DOX) or minocycline (MIN). Data plotted is Wolbachia surface protein (wsp) single gene copy number per adult female worm, horizontal bars are median levels. Shaded area indicates levels above 90% depletion compared with median vehicle control level (dashed line). Data is derived from between 10 and 40 adult female worms pooled from groups of between 4–12 mice per drug treatment. Significant differences between the 13 treatment groups were identified by Kruskal-Wallis, two-tailed 1-way ANOVA, alpha = 0.05, P < 0.0001 with Dunn’s multiple tests post-hoc comparing drug groups vs vehicle control, indicated *P < 0.05, **P < 0.01 and ***P < 0.001. (c): Effect on microfilariae (mf) production following human bioequivalent dosing of doxycycline or minocycline. Data is total mf enumerated from the infection site in groups of 4 to 12 mice per drug group. Horizontal bars are median levels. Significant differences between the 13 treatment groups were identified by Kruskal-Wallis, two-tailed 1-way ANOVA, alpha = 0.05, P < 0.0001 with Dunn’s multiple tests post-hoc comparing drug groups vs vehicle control, indicated *P < 0.05, **P < 0.01 and ***P < 0.001.