Figure 1
From: Metabolic flux profiling of MDCK cells during growth and canine adenovirus vector production
(A) Schematic representation of the experimental design consisting of parallel cultures of mock-infected and CAV2-infected MDCK cells incubated with [1,2-13C]glucose and [U-13C]glutamine, as indicated. (B) Cell growth, glucose and lactate profiles. The horizontal axis represents time after label addition in both conditions. Label was administered after 4 h of PBS or virus addition. Each time point corresponds to an average of independent replicate wells, and different time points correspond to different wells. (C) Exchange rates of main nutrients and by-products. Values represent average rates and associated standard deviations determined during the first 24 h after label administration. Es. AAs refers to the cumulative uptake of all essential amino acids measured.
