Figure 5 | Scientific Reports

Figure 5

From: Salusin-β induces foam cell formation and monocyte adhesion in human vascular smooth muscle cells via miR155/NOX2/NFκB pathway

Figure 5

Nicotinamide-adenine dinucleotide phosphate Oxidase 2 (NOX2) derived reactive oxygen species (ROS) contributed to p65-NFκB nuclear translocation and ACAT-1 and VCAM-1 transcriptions response to salusin-β in VSMCs.

(A) dose and time effects of salusin-β on the NOX2 expression. (B) effects of a free-radical scavenger N-acetylcysteine (NAC, 1 mM) on ACAT-1 and VCAM-1 expressions and p65-NFκB nuclear translocation. (C) effects of a NAD(P)H oxidase inhibitor apocynin (Apo, 100 μM) on ACAT-1 and VCAM-1 expressions and p65-NFκB nuclear translocation. The VSMCs were pretreated with NAC or Apo for 30 min and then challenged with salusin-β (30 nM) for 48 h. (D) effects of NOX2 siRNA (100 nM) on ACAT-1 and VCAM-1 expressions and p65-NFκB nuclear translocation. VSMCs were pretreated with NOX2 siRNA for 24 h and then stimulated with salusin-β (30 nM) for 48 h. Values are mean ± S.E.M. *P < 0.05 and **P < 0.01 vs. 0 h, 0 nM or PBS or Veh; †P < 0.05 and ††P < 0.01 vs. PBS+Salusin-β or Veh+Salusin-β. n = 4 for each group.

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