Figure 7

miR155 is responsible for salusin-β-induced foam formation, monocyte adhesion, NOX2 expression, ROS production, p65-NFκB nuclear translocation and ACAT-1 and VCAM-1 expressions in VSMCs.

(A) time-effects of salusin-β (30 nM) on miR155 expression; (B) effects of miR155 inhibitor on salusin-β-induced intracellular cholesterol content and monocyte adhesion. (C) effects of miR155 inhibitor on salusin-β-induced NOX2 expression; (D) effects of miR155 inhibitor on salusin-β-induced foam formation and monocyte recruitment; (E) effects of miR155 inhibitor on salusin-β-induced ACAT-1 and VCAM-1 expressions; (F) effects of miR155 inhibitor on salusin-β-induced p65-NFκB nuclear translocation. (G) representative images of Western blotting. In B-G, VSMCs were pretreated with miR155 inhibitor (100 nM) for 24 h and then stimulated with salusin-β (30 nM) for 48 h. Values are mean ± S.E.M. ^*P < 0.05 and ^**P < 0.01 vs. 0h or PBS. ^†P < 0.05 and ^††P < 0.01 vs. PBS+Salusin-β or Veh+Salusin-β.; n = 4 for each group.