Figure 2 | Scientific Reports

Figure 2

From: N-acetylaspartate catabolism determines cytosolic acetyl-CoA levels and histone acetylation in brown adipocytes

Figure 2

Aspa is expressed in brown adipose tissue and located to the cytoplasm in brown adipocytes.

(a) Aspa tissue expression in 8–10 -week old, male, ad libitum-fed C57Bl/6 mice (n = 3). (b) Male C57Bl/6J mice (age 9 months) on chow diet were challenged by cold exposure (4–8 °C) for a period 9 consecutive days. (c,d) In vitro Aspa expression during differentiation of primary brown adipocytes (n = 2, (c)) and iBACs (n = 3, (d)). (e) Western blot after fractionation of mature iBACs and BAT from 8–10 weeks old, male, ad libitum-fed C57Bl/6 mice. Beta-actin serves as cytoplasmic control protein.

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