Figure 4 | Scientific Reports

Figure 4

From: BET Bromodomain Suppression Inhibits VEGF-induced Angiogenesis and Vascular Permeability by Blocking VEGFR2-mediated Activation of PAK1 and eNOS

Figure 4

Effect of BET inhibition on VEGF-induced activation of VEGFR2 and PAK1 in HUVECs.

(A,B) Western blot analysis of VEGF-induced phosphorylation of VEGFR2 in HUVECs. The cells were pretreated with DMSO or JQ1 for 6 h or transfected with or without specific Brd2 shRNA (shBrd2) or Brd4 shRNA (shBrd4) or control shRNA (ctrl) and stimulated with 10 ng/mL VEGF for 15 min. (C,D) Western blot analysis of phosphorylated PAK1 in HUVECs pretreated with DMSO or JQ1 for 3 h (C) or transfected with Brd2 shRNA or Brd4 shRNA or control shRNA (D) in the presence or absence of VEGF (10 ng/mL). (E,F) Effect of the VEGFR2 inhibitor axitinib (E) or siRNA (F) on PAK1 activation. HUVECs were pretreated with axitinib (10 nM) for 1 h or transfected with VEGFR2 siRNA and then stimulated with 10 ng/mL VEGF for 15 min. Densitometry was performed and fold change of protein expression is shown in the right (for JQ1 or axitinib or siRNA) or lower panel (for shRNA). All values represent mean ± SEM. *P < 0.05, **P < 0.01 vs. DMSO or control siRNA or control shRNA, #P < 0.05 vs. VEGF.

Back to article page