Figure 5 | Scientific Reports

Figure 5

From: BET Bromodomain Suppression Inhibits VEGF-induced Angiogenesis and Vascular Permeability by Blocking VEGFR2-mediated Activation of PAK1 and eNOS

Figure 5

Effect of BET inhibition on VEGF-induced activation of endothelial nitric oxide synthase (eNOS) in HUVECs.

(A) Western blot analysis of VEGF-induced activation of eNOS in HUVECs. The cells, which were pretreated with DMSO or JQ1 for 6 h, were stimulated with 10 ng/mL VEGF for 15 min. (B) HUVECs were transfected with or without specific Brd2 shRNA (shBrd2), Brd4 shRNA (shBrd4), or control shRNA (ctrl) and were then stimulated with VEGF (10 ng/mL) for 15 min. (C) Effect of the VEGFR2 inhibitor axitinib on eNOS activation. HUVECs, which were pretreated with or without axitinib (10 nM) for 1 h, were stimulated with 10 ng/mL VEGF for 15 min and then lysed and analyzed by western blotting. Densitometry was performed and fold change of protein expression is shown in the right (for JQ1 or axitinib) or lower panel (for shRNA). All values represent mean ± SEM. *P < 0.05 vs. DMSO or control shRNA, #P < 0.05 vs. VEGF.

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