Figure 5
Effects induced by ENO1 are TLR4 pathway-dependent.
1.106 PBMC from healthy donors (A–C) and RA patients (D–F) were cultured with ENO1 (50 μg/mL), LPS (1 μg/mL) or BSA (50 μg/mL) with or without increasing doses of TAK242 (0, 1, 10 and 100 μg/mL). Supernatants were removed at 0–72 hours and cytokine (TNF-α and IL-10) levels were measured by ELISA. Data are expressed as mean ± SEM (n = 3). 1.106 of HEK-Blue hTLR4 cells were cultured with ENO1 (50 μg/mL), LPS (1 μg/mL) or BSA (50 μg/mL). Supernatants were removed (200 μL) at 0–72 hours and TLR4 activation was quantified by QUANTI-Blue. Data are expressed as mean ± SEM (n = 2) (G).
