Figure 2
From: Novel PKC-ζ to p47phox interaction is necessary for transformation from blebbishields
The S6K inhibitor BI-D1870 effectively blocks serum withdrawal–induced ROS with TNF-α but not with the Smac mimetic.
(a) DCF-DA FACS showing that the S6K inhibitor BI-D1870 (5 μM) inhibits serum withdrawal–induced ROS as a single agent. (b) RT4P cells were treated with BID-1870 (5 μM) with or without TNF-α or TL-32711 for 3 hours in serum-free MEM and subjected to DCF-DA FACS. Note that BI-D1870 effectively inhibits ROS with TNF-α (arrow) but not with TL-32711. (c) RT4P cells were treated with BID-1870 (5 μM) plus TNF-α with or without 100 μM H2O2 for 3 hours in 10% FBS containing MEM and subjected to DCF-DA FACS. The intensity peaks were merged with Flowjo.
