Figure 3 | Scientific Reports

Figure 3

From: Construction of recombinant baculoviruses expressing hemagglutinin of H5N1 avian influenza and research on the immunogenicity

Figure 3

The proliferation response of chicken peripheral blood lymphocyte cells.

ConA and HA protein were used to stimulate the proliferation response. The stimulation index of different regulatory elements and different promoters were compared. BV-S series (BV-S-HA, BV-S-ITRs-HA and BV-S-con-HA) refers to the recombinant baculovirus started by CMV, containing different regulatory elements of BV-S-HA (WPRE), BV-S-ITRs-HA (WPRE, ITRs), BV-S-con-HA; BV-A series (BV-A-HA, BV-A-ITRs-HA and BV-A-con-HA) refers to the recombinant baculovirus started by ie1, containing different regulatory elements of BV-A-HA (WPRE), BV-A-ITRs-HA (WPRE, ITRs), BV-A-con-HA. BV-con refers to baculovirus without any target gene, vaccine refers to immunized with commercial inactivated AIV vaccines and PBS refers to control which immunized with PBS. The stimulation index of BV-A series group started by WSSV ie1 promoter significantly higher than BV-S series droved by CMV promoter and the vaccine group. As for the group containing the same promoter, baculovirus with WPRE and ITRs regulatory elements were significant higher than baculovirus not containing any element.

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