Figure 2
Characterization of AtSWEET4.
(A) Relative expression levels of AtSWEET4 in wild-type Col-0, RNAi4-8 and OE4-4 plants detected by qRT-PCR. (B) Different sizes of wild-type Col-0, RNAi4-8 and OE4-4 seedlings grown in soil under normal conditions for 5 days. (C) Phenotype of F1 seedlings from the cross of atsweet4-3 and Col-0. (D) Phenotype of F1 seedlings from the cross of Col-0 and RNAi4-8. (E) Phenotypes of mature leaves detached from Col-0, RNA4-8, OE4-4, atsweet4-3 and F1’s of atsweet4-3 × Col-0 and RNAi4-8 × Col-0. (F) qRT-PCR analysis of AtSWEET4 expression in Col-0 and F1’s from crosses of atsweet4-3 × Col-0 and RNAi4-8 × Col-0. (G) Expression levels of six other AtSWEET genes in Col-0, RNAi4-8 and atsweet4-3 plants. Data represent the mean ± SE (n = 3). **p value < 0.01, t-test. (H,I) Chlorophyll a content (H) and Chlorophyll b content (I) in Col-0, AtSWEET4-transgenic lines and atsweet4-3 plants. Fully expanded rosette leaves were collected at the daytime from each type of plants and weighed for 500 mg, strips of leaves were used for chlorophyll measurement and three replicates were performed. Data represent the mean ± SE (n = 3). *p value < 0.05, t-test. (J,K) The expression levels of glutamate semialdehyde aminotransferase 1 gene (GSA-AT1) (J) and GSA-AT2 (K) in Col-0, RNAi4-8, OE4-4 and atsweet4-3 plants. Data represent the mean ± SE (n = 3). *p value < 0.05, t-test. Scale bars: 1 mm (B–D) and 1 cm (E).
