Figure 3
AtSWEET4 mediates glucose and fructose transport.
Glucose (A) and fructose (B) contents in the leaves of wild-type Col-0, transgenic lines and atsweet4-3 plants. Fully expanded rosette leaves were collected at the end of night and weighed for 50 mg, extracted with extraction buffers and then performed for gas chromatograph-mass spectrometry (GC-MS) analysis. Each analysis was performed three times with similar results. The data represent the mean ± SE (n = 3). *p value < 0.05, **p value < 0.01, t-test. (C) Complementation of fructose and glucose transport deficiency by AtSWEET4 in yeast EBY.VW4000 strain. (D–G) Germination rates of Col-0, RNAi4-8 and OE4-4 on 1/2 MS (1/2 MS salts) media supplemented with no sugar (D), 1% sucrose (E), 1% fructose (F) and 1% glucose (G). Sixty seedlings were counted for each plant. (H–K) Phenotypes of seedlings of Col-0, RNAi4-8, OE4-4 and atsweet4-3 on 1/2 MS media supplemented with 1% fructose (H), 1% glucose (I), 6% fructose (J) and 6% glucose (K). (L) Responses of seedlings to ABA, seedlings of Col-0, RNAi4-8, OE4-4 and atsweet4-3 were grown on 1/2 MS media supplemented with 1 μM ABA. Scale bars: 0.5 cm.
