Figure 2
From: Crypt cells are involved in kin recognition in larval zebrafish
Effect of exposure duration of different stimuli on activity of cOSNs, mOSNs and crypt cells.
9 day old zebrafish larvae were exposed to either food odor, non-kin larvae odor or E3 medium as control (ctr) for either 3, 7, 11, or 15 minutes (min). The total number of pERK+activated cOSNs, mOSNs and crypt cells was counted per larva and statistically analyzed. Box plots show median, upper and lower quartile and whiskers (maximum interquartile range: 1.5). (a) Stimulus duration does not affect number of pERK + cOSNs in larvae stimulated with food odor (Kruskall-Wallis test: H(2) = 0.794, p = 0.851, n3,7,11 min = 5, n15 min = 3), larvae odor (H(2) = 3.030, p = 0.387, n3,15 min = 5, n7,11 min = 4), or in controls (H(2) = 2.866, p = 0.413, n3,7,11,15 min = 5). (b) Number of pERK + mOSNs does not alter at different stimulus durations when stimulated with food odor (H(2) = 1.714, p = 0.634), larvae odor (H(2) = 0.964, p = 0.810), or in controls (H(2) = 4.779, p = 0.189). For n values: see (a). (c) No significant difference in number of pERK+ crypt cells at different stimulus durations when stimulated with food odor (H(2) = 2.488, p = 0.478), larvae odor (H(2) = 6.685, p = 0.083), or in controls (H(2) = 6.316, p = 0.097). For n values: see (a).
