Figure 7: CHIKV presence in saliva and virus titres of saliva. | Scientific Reports

Figure 7: CHIKV presence in saliva and virus titres of saliva.

From: Infection pattern and transmission potential of chikungunya virus in two New World laboratory-adapted Aedes aegypti strains

Figure 7

(a) CHIKV titres in head and thorax tissues (including salivary glands) from HWE and ORL mosquitoes at 2, 4 and 7 dpi. Virus titres were analysed by plaque assay in Vero cells. Saliva samples were collected from individual females prior to dissecting their head/thorax tissues. Each data point represents the CHIKV titre in a single head/thorax (and saliva) sample. Bars represent medians. (b) Number of saliva samples from HWE (n = 20) and ORL (n = 20) mosquitoes collected at 2, 4, and 7 dpi (shown in Fig. 7a) producing CHIKV-typical CPE in inoculated Vero cells. The colour code shows how many saliva samples out of a total number of 20 (per mosquito strain and collection day) caused CPE in Vero cells at 1, 2, 3, 5 and 7 days post-inoculation as an indication for low/high virus titre in a sample. (c) Maximal CHIKV titres in saliva samples collected from individual HWE and ORL females at 2, 4, 7 dpi.

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