Table 1 PCR primer sequences and product length.
Gene | Abbr. | Forward primer | Reverse primer | Size of product |
|---|---|---|---|---|
Alpha galactosyltransferase | α-gal | 5′-GCTCCACCTGGCAGTCATAG-3′ | 5′-GTCCTGGAGGATTCCCTTGA-3′ | 361 bp |
Porcine endogenous retrovirus | PERV | 5′-CTACCCCGAGATTGAGGAGC-3′ | 5′-GGGGGATGGTTAGTTTTCCA-3′ | 317 bp |
Swine leukocyte antigen DR alpha | SLA-DRα | 5′-CGAGAAGAGGTGGCAAGACA-3′ | 5′-GTCCTGGAGGATTCCCTTGA-3′ | 220 bp |
Swine leukocyte antigen 2 | SLA-2 | 5′-GTCACCTTGAGGTGCTGGG-3′ | 5′-TGGCAGGTGTAGCTCTGCTC-3′ | 185 bp |
