Figure 1: Plant transformation vector details and molecular confirmation of transgene integration and its expression.
From: The development of a phosphite-mediated fertilization and weed control system for rice
(a) Schematic representation of T-DNA region of plant transformation vector (pMDC99) showing the ptxD gene expression cassette consisting of Right Border (RB), rice Actin2 promoter (Os Act2P), phosphite dehydrogenase gene (ptxD), rice Actin2 terminator (Os Act2T), 35S promoter (35SP), hygromycin phosphotransferase gene (hpt+), PolyA (PolyA) and LB Left Border (LB). (b) Southern blot showing Sac II digested genomic DNA from wild type (WT) and transgenic rice lines (1–17) probed with DIG labeled hpt gene. (c) Northern blot revealing the ptxD transcript from of T1 transgenic and WT plants. (d) Semi quantitative RT-PCR revealing expression of ptxD and Actin1 gene in transgenic and WT plants. M indicates 100 bp plus DNA ladder.
