Figure 1 | Scientific Reports

Figure 1

From: The intracellular trafficking mechanism of Lipofectamine-based transfection reagents and its implication for gene delivery

Figure 1

(A) Schematic evaluation of a single particle track from a set of confocal images acquired within 300 s, with a time lapse Δt = 1 s. Representative trajectories of complexes in not treated chinese hamster ovarian (CHO) cells: (B) Lipofectamine/DNA; (C) DOTAP/DOPC/DNA (DD/DNA). Representative trajectories of complexes in nocodazole (NCZ)–treated CHO cells: (D) Lipofectamine/DNA and (E) DD/DNA. Diffusion (red) and flow motion (blue) segments are shown. Relative populations of the acquired tracks for Lipofectamine and DD in not treated (F) and NCZ-treated (G) CHO cells. (H) Mean square displacement (MSD) analysis of two representative tracks. MSD calculation was used for the measurement of the dynamic parameters, i.e. diffusion coefficients and flow speed.

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