Figure 2: Analysis of uterine expression for Cbx2, Cbx4, Ring1B, and Rybp during early pregnancy at protein levels.

(A) Western blot analysis. Total protein extracts of uterine tissues were collected during early pregnancy on D4, D5-IS, D7-IIS, and D7-IS. Experimentally induced deciduoma and control horns were also analyzed on D7 pseudopregnancy. Ut, uterus; IS, implantation site; IIS, interimplantation site; Co, control; DM, deciduoma. (B) Quantitation was achieved by direct analysis of the bands from (A). Fold changes in protein levels were normalized by Actin. *Values are statistically different (P < 0.05). (C) IHC localization of Cbx2, Cbx4, and Rybp expression. Representative uterine tissue sections on D4, D5-IS, and D7-IS in the M- and AM-pole locations are shown. Sections are shown at 100X and 400X (as insets). Red staining indicates the localization of immunoreaction. le, luminal epithelium; ge, glandular epithelium, s, stroma; ds, decidualizing stroma; pdz, primary decidual zone; sdz, secondary decidual zone; dec, decidual cells; e, embryo; tgc, trophoblast giant cells; M, mesometrial pole; AM, antimesometrial pole. Arrow indicates polyploid cells. (D) IF analysis of Ring1B. Blue and green staining indicates the localization of DAPI (for nuclear) and Ring1B, respectively. Sections are shown at 100X and 400X (as insets). These experiments were repeated at least three times with similar results.