Figure 2: Biochemical evaluation by in vitro kinase assay of the FBS hits (F1 and F2) and their analogues (F1.1 to F1.9, and F2.1 to F2.10). | Scientific Reports

Figure 2: Biochemical evaluation by in vitro kinase assay of the FBS hits (F1 and F2) and their analogues (F1.1 to F1.9, and F2.1 to F2.10).

From: Putative histidine kinase inhibitors with antibacterial effect against multi-drug resistant clinical isolates identified by in vitro and in silico screens

Figure 2

(A) First, the autophosphorylation inhibitory activity was evaluated in a one concentration (2mM) one time-point (30 sec) in vitro kinase assays with WalK and PhoRE. The fragments inhibited WalK and PhoRE autophosphorylation with 10 to 62% and 17 to 80%, respectively. (B) IC50 of the more potent inhibitors (% inhibition at 2 mM > 50%) with antibacterial effect were measured in a multiple-concentrations one time-point (30 sec) experiments.

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