Figure 5
From: Involvement of the P2X7-NLRP3 axis in leukemic cell proliferation and death
NLRP3 overexpression in Ramos cells triggers cell death.
Ramos cells were transfected with a plasmid encoding NLRP3 as described in Methods and then analyzed for NLRP3 mRNA and protein expression (a,b) Empty vector (mock)-transfected cells and a HEK293 clone stably transfected with NLRP3(HEK293NLRP3) are shown as control. Effect of NLRP3 over- expression on caspase-3 mRNA (c) and protein (e) expression and caspase-3 activation (d) is shown. Effect of NLRP3 transfection on caspase-9 expression (f) and apoptotic cell number (annexin V-positive cells) is shown in panel (g). Data are averages ± SEM from 3 to 4 independent determinations. *p < 0.05; ***p < 0.001.
