Figure 2 | Scientific Reports

Figure 2

From: Rapid and sensitive detection of early esophageal squamous cell carcinoma with fluorescence probe targeting dipeptidylpeptidase IV

Figure 2

Fluorescence imaging of human esophageal squamous cell carcinoma cell line KYSE270 with GP-HMRG.

(a) In vitro changes in absorption (left) and fluorescence (middle) spectra of GP-HMRG before and after addition of DPP-IV. (b) Confocal fluorescence images at 5 and 60 min after incubation with GP-HMRG (10 μM) in the absence and presence of inhibitor (100 μM). Drastic fluorescence activation of GP-HMRG was observed and was significantly suppressed by the inhibitor. Scale bars, 100 μm. (c) Confocal fluorescence images of KYSE270 cells which had been pretransfected with siRNAs, followed by application of GP-HMRG (10 μM). Scale bars, 100 μm. (d) Change of fluorescence intensity in KYSE270 cells in (b). Data are mean fluorescence intensities (a.u.) ± SEM.

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