Figure 2
From: Small molecule activation of NOTCH signaling inhibits acute myeloid leukemia
NMHC induced cell cycle arrest and cell apoptotic death in AML.
(a) Analysis of cell cycle distributions in NMHC-treated cells. Cells were treated with various doses of NMHC for 24 h, followed by cell cycle analysis. The percentages of G0-G1 phases are shown in each graph. Effect of NMHC on cell cycle-relevant proteins was shown in the bottom. Cyclin B1 and p21 were detected by immunoblotting after 24 h treatments. (b) Analysis of apoptotic cell death. Cells were treated with various doses of NMHC for 24 h, stained with Annexin V-FITC/PI and analyzed by flow cytometry. Apoptosis-relevant proteins PARP and cleaved Caspase-3 were assessed by immunoblotting after DMSO or NMHC (1 μM) treatments for 24 h. Above all, representative data from three independent experiments are shown. β-actin is detected as the internal reference.
