Figure 6 | Scientific Reports

Figure 6

From: The independent contribution of miRNAs to the missing heritability in CYP3A4/5 functionality and the metabolism of atorvastatin

Figure 6

MiR-27b represses CYP3A activity by directly binding with CYP3A4 3′-UTR.

(a) The predicted target sequence of miR-27b in human CYP3A4 mRNA. (b) The potential miR-27b recognition element with blue base (CYP3A4 MRE 27b) is located between bases 2226 and 2232. (c) The wild type or mutant reporter plasmids (100 ng) were co-transfected with miR-27b mimic or control into HEK293 cells. The Renilla luciferase activity for each construct was normalized with the firefly luciferase activity. (d) The effect of miR-27b mimic or inhibitor on CYP3A activity. (e) Relative CYP3A4 mRNA level. (f) Relative CYP3A5 mRNA level. The values of activity and mRNA level were measured 48 h after transfection with mimic or inhibitor for miR-27b. Each column represents the mean ± SD of three independent experiments. *P < 0.05, **P < 0.001, ***P < 0.001.

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