Figure 2 | Scientific Reports

Figure 2

From: Honokiol suppresses TNF-α-induced neutrophil adhesion on cerebral endothelial cells by disrupting polyubiquitination and degradation of IκBα

Figure 2

Honokiol suppressed TNF-α-induced adhesion molecule expression.

(A) RT-qPCR analysis of vascular cell adhesion molecule-1 (VCAM-1) mRNA level in bEnd.3 ECs incubated with DMSO or honokiol (1 μM) for 30 min, then stimulated with TNF-α (10 ng/ml) for 1, 3 and 6 h. Normalization was to β-actin mRNA level. (B) RT-qPCR analysis of VCAM-1 mRNA level in ECs incubated with honokiol at 1, 3 and 10 μM for 30 min, then stimulated with TNF-α (10 ng/ml) for 1 h. (C) ECs were incubated with DMSO or honokiol (3 or 10 μM) for 30 min, then stimulated with TNF-α (10 ng/ml) for 1 h. ECs were stained with rat IgG or anti-VCAM-1antibodies for 30 min. The unbound antibodies were removed and then Alexa Fluor 488 anti-rat IgG was added for 30 min. Fluorescein-labelled ECs were analysed for surface VCAM-1 expression by flow cytometry. ECs incubated with rat IgG and Alexa Fluor 488 dye-conjugated antibodies were rat IgG controls. Data are mean  ±  SEM from 3 independent experiments. *P < 0.05 vs untreated control. #P < 0.05 vs corresponding control (TNF-α alone).

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