Table 2 Mutation rate of T0 regenerated tobacco plants in the target gene.
From: Highly specific targeted mutagenesis in plants using Staphylococcus aureus Cas9
target gene | Cas9 | No. of plants examined | No. of plants with mutations | Mutation rate (%) |
|---|---|---|---|---|
NtPDS | SaCas9 | 45 | 34 | 75.6 |
SpCas9 | 44 | 35 | 79.5 | |
NtFT4 | SaCas9 | 43 | 28 | 65.1 |
SpCas9 | 22 | 13 | 59.1 |