Table 2 Mutation rate of T0 regenerated tobacco plants in the target gene.

From: Highly specific targeted mutagenesis in plants using Staphylococcus aureus Cas9

target gene

Cas9

No. of plants examined

No. of plants with mutations

Mutation rate (%)

NtPDS

SaCas9

45

34

75.6

 

SpCas9

44

35

79.5

NtFT4

SaCas9

43

28

65.1

 

SpCas9

22

13

59.1